Next Generation Histology

Efficient cleavage and probe versatility for highly-multiplex detection and co-localization

Modified Lightning Terminators provide a robust method for detecting multiple antigens from a single sample.  Immunofluorescent detection of antigens in fixed tissue specimens and on immunoblots is used routinely in clinical practice and research laboratories, but current methods are limited to the detection of one to four antigens per tissue section or blot. Consequently, the detection of additional antigens requires multiple independent stains on separate sections and limits the co-localization of antigens on a single section.  Whether in immunohistochemistry or immunoblotting, our photocleavable fluorescent labels (PCLs) provide advantages over current methods.

Highly Serial and Multiplex Detection

PCL technology uses our expertise in photocleavable chemistry to provide structures that can both efficiently bind to target antigens and rapidly cleave the dye reporter after exposure to UV light. The method results in high signal detection of multiple antigens from a single sample with negligible sample damage between stains. Preliminary results show successful biomarker detection on biopsy tissue sections through multiple stains and indicate the potential for detection of more than 20 antigens on a single slide.  Additional work with dot blots indicate the potential of PCLs in blotting methods ranging from Western blots to Reverse Phase Protein Array (RPPA).

Versatility for Compatibility with Standard Methods

The Lightning Terminators used for target detection can be provided in multiple configurations to meet the needs of researchers.  The probe type, such as antibody or biotin, can be easily customized to work with current methods.  Additionally, the choice of dye label can be modified to provide multiple fluorescent wavelengths for detection with different microscopes. The probes can be used in manual workflows or with the automated NGH platform that is currently under development.